Genetic introgression of escaped farmed Atlantic salmon ( Salmo salar ) into wild populations is a major environmental concern for the salmon aquaculture industry. Using sterile fish in commercial aquaculture operations is, therefore, a sustainable strategy for bio-containment. So far only the methodology used commercially for producing sterile salmon is triploidization; however, triploid fish are less robust. A novel approach to achieve sterility is to produce germ cell-free salmon. One way to accomplished this is to knock out the dnd (dead end ) gene using CRISPR-Cas9. The lack of germ cells in the resulting dnd crispants , thus, prevents reproduction and subsequent large-scale production of the sterile fish. We have therefore sought to develop an approach suitable to obtain sterility, but combined with a method to inherit sterility in dnd knockout broodstock (Patent WO2020/070105): Inheriting sterility in broodstock salmon can be achieved by rescueing dnd gene function (by dnd mRNA co-injection) of dnd crispant salmon embryos. In one-year old rescued dnd crispant salmon we found germ cells, type A spermatogonia in males and previtellogenic primary oocytes in females. We have further followed rescued dnd crispants through oogenesis and spermatogenesis and obtained an F1 generation from incrosses . This method allows the large-scale production of Atlantic salmon broodstock that can inherit the sterility trait, but are able to produce 100% germ-cell free offspring. T here may be partial, but not critical, limitations of the method due to potential functions of dnd during gametogenesis, something we are currently exploring in rescued F1 fish showing complete or partial dnd loss-of function, and in gametes obtained from rescued F0 crispants . This approach may solve the problems of both, genetic introgression and pre cocious maturation in farmed salmon, while ensuring a stable production of 100% sterile fish, and thus represents a significant commercial potential. Use of this sterility technology may also pave the way for safe genome editing of other traits, such as disease resistance, which would be contained in sterile individuals and hence presented a negligible risk of passing edited alleles on to wild stocks.