Aquaculture Europe 2023

September 18 - 21, 2023

Vienna,Austria

Add To Calendar 21/09/2023 16:15:0021/09/2023 16:30:00Europe/ViennaAquaculture Europe 2023BENEFICIAL EFFECTS OF A MARINE BACILLUS MULTI-STRAIN CONSORTIUM ENCAPSULATED IN ALGAE ON GROWTH PERFORMANCE, DIGESTIVE AND IMMUNITY GENE EXPRESSIONS, VIBRIO RESISTANCE, MICROBIOTA MODULATION AND TRANSCRIPTOME PROFILING OF WHITE SHRIMP Penaeus vannameiSchubert 1The European Aquaculture Societywebmaster@aquaeas.orgfalseDD/MM/YYYYaaVZHLXMfzTRLzDrHmAi181982

BENEFICIAL EFFECTS OF A MARINE BACILLUS MULTI-STRAIN CONSORTIUM ENCAPSULATED IN ALGAE ON GROWTH PERFORMANCE, DIGESTIVE AND IMMUNITY GENE EXPRESSIONS, VIBRIO RESISTANCE, MICROBIOTA MODULATION AND TRANSCRIPTOME PROFILING OF WHITE SHRIMP Penaeus vannamei

FOODLAND and PrAEctiCe are European funded Horizon projects focusing on food production and farming in Africa. FOODLAND began in September 2020 and will run until August 2024 aiming to enhance the diversity of food production and consumption in six African countries displaying different stages of the nutrition transition. To this end, FOODLAND will create a network of 14 local Food Hubs that will aggregate relevant actors and serve as injection points for the introduction of innovations. FOODLAND has identified specific objectives addressing the organizational, technological, and nutritional needs of the local African food systems: 1. To detect behaviour and preferences of consumers and producers, in order to customize innovations to local sensitivities; 2. To develop and implement organizational innovations, aimed at boosting coordination among food operators; 3. To develop, test, and validate (open) technological innovations in the laboratory and in the field; 4. To disseminate knowledge of solutions towards malnutrition reduction and innovations.

PrAEctiCe began in November 2022 and is a 42-month project that will provide a novel agroecology indicator set for East Africa, aimed at helping smallholder farmers in their agroecological transition. The project goes beyond the existing indicator frameworks by putting the “concept into action” with a decision support tool for agroecology advisors supporting the selection of the best suited combination of agroecological farming practices in a local context.

These projects give strong attention to activities pertaining to aquaculture. FOODLAND is developing aquaculture technologies for urban and peri-urban areas to ensure the production is brought closer to the markets resulting in a shorter distribution chain that can be more competitive with imported products. The Aquaculture Working Group, based in Kenya, Uganda, Tanzania, and Tunisia, are innovating in several different areas to develo

Introduction

Aquaculture production is increasingly looking towards innovative solutions to further improve gut health and production sustainability. Marine probiotics have the potential to support the control of infections and to improve zootechnical performances, while minimizing the environmental impact. We investigated the probiotic potential of Bacillus multi-strain consortium extracted from the marine environment and encapsulated in algae on white shrimps, Penaeus vannamei.

Materials and methods

20-day and 60-day feeding trials were conducted respectively on healthy Penaeus vannamei PL10 (n=3500, triplicate) and PL25 (n=350, triplicate) to evaluate the effects on growth and health parameters of a marine probiotic consortium (MPC) made of four marine Bacillus strains encapsulated in algae when used as dietary supplement.

Results

At the end of the 60-day trial, the treatment with MPC at 0.1% significantly improved growth performance (final body weight, weight gain, specific growth rate) and significantly decreased feed conversion ratio of White Shrimp P. vannamei (P < 0.05). Supplementation with MPC at 0.1% also significantly decreased the occurrence of total Vibrio spp. count in shrimp’s hepatopancreas under normal conditions (P < 0.05). Overall, MPC significantly enhanced the mRNA expressions of (1) digestive genes in hepatopancreas, such as Trypsin, α-Amylase, Triacylglycerol lipase and Chymotrypsin BII; (2) immune-related genes, such as proPO, crustin genes in hepatopancreas and Dual Oxidase, mucin-like peritrophin, Penaeidin-3α in intestine genes (P < 0.05) ), especially with MPC at 0.25%. Under challenge conditions, MPC significantly increased disease resistance of shrimp larvae against V. parahaemolyticus (VpAHPND) (P < 0.05).

The results of high-throughput sequencing showed a significant improvement in White Shrimps digestive tract bacterial communities when supplemented with probiotics. Moreover, the MPC enhanced the hepatopancreas gene pathway related to metabolisms of endocrine, immune and digestive systems. In addition, the beneficial effects observed in intestine gene pathway were mainly related to translation, transport catabolism, and signal transduction.

Conclusion

This study demonstrated the ability of the marine Bacillus spp. consortium encapsulated in algae to improve White Shrimp growth performance, digestive tract & immune status and disease resistance during both larval and grow-out phases.

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