Aquaculture Europe 2023

September 18 - 21, 2023


Add To Calendar 19/09/2023 11:45:0019/09/2023 12:00:00Europe/ViennaAquaculture Europe 2023VALIDATION OF A METHODOLOGY FOR APPARENT NUTRIENT DIGESTIBILITY AND FAECAL COLLECTION BY SEDIMENTATION IN PIKEPERCH Sander luciopercaSchubert 4The European Aquaculture Societywebmaster@aquaeas.orgfalseDD/MM/YYYYaaVZHLXMfzTRLzDrHmAi181982


E. Panana* and S. Teerlinck

 Inagro,  Practical Research Aquaculture Center,  Ieperseweg 87 8800 Rumbeke- Beitem (Belgium)




In aquaculture, the evaluation of feed ingredients is essential for the development of high quality aquafeeds.  The determination of nutrient digestibility is the first step in evaluating the potential of an ingredient for use in the diet of an aquaculture species.  This information  is very useful  for formulation of diets that maximize the growth of fish, by providing appropriate amounts of available nutrients, but also to limit fish waste products.

 Over the last decades, apparent digestibility coefficients (ADC) of dry matter, crude protein, crude lipid, gross energy and phosphorus of different ingredients have been determined  for different aquaculture species. However, different methods of feces collection ( dissection, stripping or sedimentation) and different  inert  markers (c hromic oxides,  celites or  yttrium) have been used, which  often makes it difficult to compare results. Moreover,  there is a lack of information on the  ADC of ingredient in emerging aquaculture species. To our knowledge, n o reports are available for the ADC of nutrients in pikeperch (Sander lucioperca) . The objective of this work  was  to validate feces collection by sedimentation for the determination of ADC in macronutrients and phosphorus in pikeperch using  yttrium as inert market.

Material and methods

The tria ls were conducted in a recirculating aquaculture system (RAS) consisting of 12 circular tanks (750-L/tank), a drum filter, moving bed filter, UV filter and an oxygen tower. Each tank was connected to a 200-L se ttler for feces collection .

A practical diet , 52% crude protein, 17% crude  fat, 20.5 MJ/kg gross energy and 0.02% Yttrium (Y2O3) as inert market, was used in two trials. In the first trial, fifty p ikeperch (b ody weight: 109 ± 9.8 g )  were stocked on each tank for ADC determination. For three weeks, p ikeperch  were  fed  once a day until apparent satiation. The  feces  were collected after 18 hours,  in  cold plastic tubes  connected to the bottom of the settlers, and stored  at  -20°C until analysis . The feed and the feces were analyzed for proximal composition of macronutrients, phosphorus and yttrium.  The ADC was calculated  using the formulas described by


 In the second trial,  feces collection  using three different  strategies was determined. 1 )  one  feeding per day- feces collection after  18 hours; 2) one feeding per day -feces collection after 21 hours; 3) two feedings per day-two feces  collection: after 6 hours and  after 18hours.  The percentage of feces recovery; feces production and dry weight of feces were determined.

 The  results  of  both trials  will be presented and discussed during the EAS2023 percid session.


Glencross BD, Booth M, & Allan GL. (2007). A feed is only as good as its ingredients – a review of ingredient evaluation strategies for aquaculture feeds. Aquaculture nutrition, 13, 17-34.